RESUMO
INTRODUCTION AND AIMS: Colorectal cancer (CRC) is the third most prevalent cancer worldwide. Many risk factors are involved, and current evidence links the gut microbiota and colorectal carcinogenesis. Fusobacterium nucleatum (F. nucleatum) is proposed as one of the risk factors at the onset and during the progression of CRC, due to immune system and inflammatory modulation. MATERIALS AND METHODS: Ninety samples from three different regions of the colon were collected through colonoscopy in patients with CRC, and qPCR TagMan® was conducted to detect F. nucleatum and cytokines (IL-17, IL-23, and IL-10) in tumor, peritumor, and normal samples. The differences between them were analyzed and correlated. RESULTS: The abundance of F. nucleatum determined through the 2-ΔΔCt method in CRC (7.750 [5.790-10.469]) was significantly higher than in the normal control (0.409 [0.251-0.817]) (p < 0.05). There was no significant association between F. nucleatum and the cytokines (p > 0.05). CONCLUSIONS: CRC is a heterogeneous disease that presents and progresses in a complex microenvironment, partially due to gut microbiome imbalance. F. nucleatum was enriched in CRC tissue, but whether that is a cause of the pathology or a consequence, has not yet been clearly defined.
Assuntos
Neoplasias Colorretais , Infecções por Fusobacterium , Carcinogênese , Neoplasias Colorretais/diagnóstico , Neoplasias Colorretais/epidemiologia , Neoplasias Colorretais/etiologia , Citocinas , Infecções por Fusobacterium/complicações , Infecções por Fusobacterium/epidemiologia , Fusobacterium nucleatum , Humanos , Microambiente TumoralRESUMO
INTRODUCTION AND AIMS: Colorectal cancer (CRC) is the third most prevalent cancer worldwide. Many risk factors are involved, and current evidence links the gut microbiota and colorectal carcinogenesis. Fusobacterium nucleatum is proposed as one of the risk factors at the onset and during the progression of CRC, due to immune system and inflammatory modulation. MATERIALS AND METHODS: Ninety samples from three different regions of the colon were collected through colonoscopy in patients with CRC, and qPCR TagMan® was conducted to detect F. nucleatum and cytokines (IL-17, IL-23, and IL-10) in tumor, peritumor, and normal samples. The differences between them were analyzed and correlated. RESULTS: The abundance of F. nucleatum determined through the 2-ΔΔCt method in CRC (7.750 [5.790-10.469]) was significantly higher than in the normal control (0.409 [0.251-0.817]) (p<0.05). There was no significant association between F. nucleatum and the cytokines (p>0.05). CONCLUSIONS: CRC is a heterogeneous disease that presents and progresses in a complex microenvironment, partially due to gut microbiome imbalance. F. nucleatum was enriched in CRC tissue, but whether that is a cause of the pathology or a consequence, has not yet been clearly defined.
RESUMO
El resultado de la ligadura bilateral de las arterias hipogástricas es bueno y eficaz en el control de la hemorragia posparto para la preservación del útero, pero se han publicado pocos casos y la incidencia es baja. En el reporte de este caso, la paciente presentaba menometrorragia de dos años de evolución, sin mejoría a tratamiento médico convencional, por lo que se realizó ligadura bilateral de arterias hipogástricas y tronco posterior bilaterales, obteniendo una adecuada mejoría. La ligadura de tronco posterior bilateral es un procedimiento que se realizó de manera complementaria a la ligadura de las arterias hipogástricas y podría redundar en disminución del tiempo quirúrgico y riesgo de sangrado en las pacientes.(AU)
Although the outcome of bilateral hypogastric artery ligation is good, and effective in controlling postpartum bleeding for the preservation of the uterus, few cases have been published, and the incidence is low. In this case report, the patient presented with menometrorrhagia of two years onset. With no improvement using conventional medical treatment, a bilateral ligation of hypogastric arteries was performed as well as the posterior trunk bilaterally, obtaining an adequate improvement. Bilateral posterior trunk ligation is a procedure that was performed as a complement to hypogastric artery ligation and could result in a decrease in surgical time and risk of bleeding in patients.(AU)
